Sequencing strategy of mitochondrial HV1 and HV2 DNA with length heteroplasmy

We describe a method to obtain reliable mitochondrial DNA (mtDNA) sequences downstream of the homopolymeric stretches with length heteroplasmy in the sequencing direction. The method is based on the use of junction primers that bind to a part of the homopolymeric stretch and the first 2-4 bases downstream of the homopolymeric region. This junction primer method gave clear and unambiguous results using samples from 21 individuals with length heteroplasmy in the hypervariable regions HV1, HV2 or both. The method is of special value for forensic casework, because sequencing of both strands of an mtDNA region is preferable in order to reduce ambiguities in sequence determination.     

Evaluation of Commercial Kits for Dual Extraction of DNA and RNA from Human Body Fluids, ,

STR typing of DNA evidence can identify the donor with a high power of discrimination but cannot identify the tissue origin of a body‐fluid stain. Using RNA to attribute a crime scene stain to a particular tissue may aid in reconstruction efforts. With blood from 10 donors, four DNA and RNA coextraction kits were evaluated by measuring yields and STR and mRNA profiles. T tests indicated some significant differences in kit performance. The Zymo Research ZR‐Duet^? kit performed best based on average DNA (41.4 ng) and mRNA (4.07 ng) yields and was the only kit to provide complete DNA/RNA profiles for all samples. The consistency of this kit was challenged by data from additional blood and saliva donors. Further testing is advised before a superior kit is unequivocally chosen. Stand‐alone DNA or RNA purification generally offers higher yield, but coextraction may still allow successful STR profiling and tissue source identification.     

Characteristics of Youth Remaining in Foster Care Through Age 19: A Pre- and Post-Policy Cohort Analysis of California Data

This study examined factors associated with youth remaining in extended foster care. Cohorts of youth in care in California at age 17 years were defined for the period from 2003–2012. Multivariable generalized linear models documented factors associated with remaining in care through age 19. Findings indicate that: a) the population of youth in care at age 17 has declined over time; b) youth in care at age 17 in 2012 were qualitatively different from those a decade earlier; c) a larger proportion of youth who do not exit to permanency are remaining in extended foster care; and d) although the characteristics of youth who remain in foster care as non-minor dependents continue to vary relative to those who age out, differences have diminished. Findings highlight the potential to target subgroups of youth who may benefit from the extension of care but are remaining in care at lower rates.     

Intergenerational Continuity of Risky Parenting: A Person-Oriented Approach to Assessing Parenting Behaviors

Limited research has investigated the long-term effects of childhood emotional abuse on later forms of parenting. This study utilized a person-centered approach to explore the relation between retrospectively-reported maternal childhood emotional abuse and observed parenting behaviors during a conflict discussion. Data were collected from 53 caregiver-child dyads with children ages 8–11. Results of a model-based clustering procedure (Mclust; Fraley and Raftery 2006) identified three parenting styles (negative, at-risk, positive) that were based on five observed parenting behaviors (emotion regulation, anger, hostility, psychological control, and psychological unavailability). Results indicated that higher levels of childhood emotional abuse were reported by women in the at-risk and negative parenting subgroups. Mothers in the negative parenting and at-risk parenting clusters exhibited greater levels of emotional abuse when compared to the positive parenting cluster. Possible implications are discussed, and results underscore the importance of emotionally abusive developmental experiences in the understanding of risk for maladaptive parenting behaviors.     

The moving targets of penal abolitionism: ICOPA,past, present and future

The history of the International Conference on Penal Abolition (ICOPA) is explored, assessing major trends shaping the current growth of carceral practices and offering suggestions for the future of the conference movement. The goal here is to facilitate the expansion of abolitionism by describing the changing nature of what is to be abolished. A discussion of the emergence of prison abolitionism and of the ICOPA is presented, describing the shift in focus from prison to penal abolition. Taking these developments and character into account, an abolitionist gaze must be expanded to reflect a carceral abolition project, encompassing contemporary penal abolitionism while acknowledging the proliferation of carceral practices and spaces outside the traditional penal system.     

Perceived human rights and support for new democracies: lessons from Mexico

Political elites in emerging democracies are likely to promise improvements on human rights. From an empirical perspective, however, emerging democracies tend to perform rather poorly in this domain. Given this tension between elite rhetoric and performance, it is important to examine the extent to which citizens in emerging democracies evaluate democracy and new democratic leaders' performance on the bases of their perceptions of respect for human rights. This topic remains largely unexplored and conventional wisdom suggests that economic satisfaction, not human rights concerns, drives individuals' support for democracy. We aim to fill this gap in the literature by investigating the extent to which specific and diffuse political support is related to individuals' perceptions of respect for human rights in the context of an emerging democracy. Taking advantage of two representative survey data sets from Mexico from 2003 and 2010, our empirical findings suggest that citizens are more likely to support their president, their government and democratization when they believe that human rights are respected. By examining the relationship between democracy and human rights protections at the individual level, our research is a pioneering effort to better explain the interaction between the prospects of democratic consolidation and perceptions of human rights.     

Greenland criminal justice: The adaptation of western law

The conflict which many indigenous peoples experience with Westernized systems of law has precipitated a search for alternative models of criminal justice. While the process of colonization has most often resulted in the destruction of traditional practices of social control, in Greenland an attempt was made to adapt Western law to the indigenous culture. The Greenlandic justice system has several unique attributes which have attracted the attention of indigenous peoples and governments worldwide. This article traces the origins, development and evolution of the Greenlandic Criminal Code and criminal justice system, the factors which influenced its development, and the extent to which the objectives of the architects of the systems have been met. The discussion provides key insights into the potential and limitations of adapting Western law to indigenous cultures.     

When Abuse Happens Again: Women's Reasons for Not Reporting New Incidents of Intimate Partner Abuse to Law Enforcement

Although most women abused by intimate partners experience a patterned behavior of abuse (by either the same or new partners), little is known about their decision making regarding whether to call the police for subsequent abuse. The current study found that 90 percent of women who had encountered the criminal legal system for previous intimate partner abuse victimizations did not contact the police for some or all recurrences. Qualitative analysis was conducted among a sample of 102 women regarding their reasons for not re-engaging the legal system for subsequent victimizations. The results suggested 5 overall reasons as to why women involved with the criminal legal system choose not to engage the system again.